Although luminal obstruction has traditionally been viewed as the
underlying cause of appendicitis, recent evidence has suggested that the
disease may result directly from invasion by specific pathogens, e.g.
Fusobacterium nucleatum. The purpose of this study was to survey
microbial communities within pediatric appendectomy specimens using a
culture-independent approach.
ribosomal gene sequence analysis to profile the microbiota present
within luminal fluid obtained from 22 pediatric appendectomy specimens.
These included 10 simple appendicitis cases, 5 perforated appendicitis
cases, 2 interval appendectomies, and 5 incidental appendectomies.
could be divided into 2 distinct clusters based upon the composition of
the appendiceal bacterial communities. Appendicitis samples contained
an increased abundance of Fusobacterium spp. and a reduced abundance of
Bacteroides spp. relative to non-appendicitis cases. Appendicitis
samples also contained variable amounts of other oral taxa such as
Porphyromonas, Parvimonas, and Gemella, whereas these taxa were
generally absent from non-appendicitis samples.
appendicitis is associated with an abundance of Fusobacterium spp. and
other pathogens commonly found in the oral cavity. Further research is
needed to determine whether these organisms directly cause appendicitis
or rather proliferate in the appendix as a secondary consequence of
inflammation.
underlying cause of appendicitis, recent evidence has suggested that the
disease may result directly from invasion by specific pathogens, e.g.
Fusobacterium nucleatum. The purpose of this study was to survey
microbial communities within pediatric appendectomy specimens using a
culture-independent approach.
METHODS:
We performed 16Sribosomal gene sequence analysis to profile the microbiota present
within luminal fluid obtained from 22 pediatric appendectomy specimens.
These included 10 simple appendicitis cases, 5 perforated appendicitis
cases, 2 interval appendectomies, and 5 incidental appendectomies.
RESULTS:
Samplescould be divided into 2 distinct clusters based upon the composition of
the appendiceal bacterial communities. Appendicitis samples contained
an increased abundance of Fusobacterium spp. and a reduced abundance of
Bacteroides spp. relative to non-appendicitis cases. Appendicitis
samples also contained variable amounts of other oral taxa such as
Porphyromonas, Parvimonas, and Gemella, whereas these taxa were
generally absent from non-appendicitis samples.
CONCLUSIONS:
Acuteappendicitis is associated with an abundance of Fusobacterium spp. and
other pathogens commonly found in the oral cavity. Further research is
needed to determine whether these organisms directly cause appendicitis
or rather proliferate in the appendix as a secondary consequence of
inflammation.
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