: "While DNA methylation is an extensively-studied epigenetic modification, relatively little is known about the methylation of cytosine bases in RNA. Although the functional consequences of RNA modifications are not clear, RNA molecules are known to be extensively modified. In fact, there are over 100 reported modifications to RNA, which is even more than are currently known for DNA. Recently, Hussain et al. developed a new technique for site-specific detection of 5-methylcytosine in RNA, which they coined Methylation individual-nucleotide-resolution CrossLinking and ImmunoPrecipitation, or miCLIP. The authors employed their miCLIP method to explore the targets of NSun2 and found that tRNAs, mRNAs, and ncRNAs were all targets of this protein. They confirmed their findings using other available techniques to detect cytosine methylation in RNA, such as bisulfite conversion and 5-azacytidine-mediated immunoprecipitation, validating the miCLIP metho"
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